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Friday 12 August 2011

ISOLATION OF SPECIFIC DNA

All fragments of DNA are obtained through gene cloning. Desired fragments of DNA is obtained ad follows :-
(i) Genomic library :-
Genomic library contains all segments of DNA i.e. is contain coding and non-coding both regions of DNA,on the other hand DNA libraies consists of only coding sequences of the total genomic content.
(ii) cDNA library :-
A cDNA is a population of bacterial transferrmants or phage lysate in which each mRNA
Is islated from an organism or tissue is represented as its cDNA insertion in a plasmid o a phage vector.

cDNA library is preferred over cienomic library.

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