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Friday 12 August 2011
DNA cloning invoves 5 general procedures
1. Cutting DNA at precise locations.
2. Joining two DNA fragments covalently.
3. Selecting a small molecule of DNA capable of self replication. Segments of DNA to be cloned can be fromed to plasmid or viral DNAs.
4. Transfer of recombinant DNA to a host cell.
5. Selection or identification of host cell that contain recombinant DNA.
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