ENZYMES USED IN RECOMBINANT DNA TECHNOLOGY

1. Alkaline phosphatase :-
Removes phosphate gps from 5’ end of ds DNA or RNA.

2. DNA ligas :
Join DNA molecules by forming phosphodiester linkage beween DNA
segments.

3. DNA polymerase 1 :
Synthesizes DNA complentary to a DNA template.

4. Dnase 1 :
Produces singel starnded nicks in DNA.

5. Reverse tansciptase :
Synthesizes DNA from RNA.

6. Terminal Transeferase :
Addu nuclecotides to 3’-ends of DNA or RNA useful in homopholymer tailing.The Classes of enzyme lie at the center of the general appoach to generating and propagating a recombinant DNA molecule.

7. SI nuclease:
Degrade ss DNA & RNA.